CAR T cells can respond very differently to identical stimuli, and build up synapses of highly heterogeneous phenotypes. We will develop a microfluidic biochip prototype allowing for highly automated analysis of individual cells, including targeted delivery to a microscope platform, characterization by fluorescence microscopy and removal of interesting cells for further characterization by other techniques. The project includes design of the biochip and i/o system, mastering of the biochip, hot embossing into thermoplastics, integration of the biochip in the i/o system and microscopy platform, and the ultimate application for our cell biological experiments.